Fig. 2
Amitriptyline inhibits acetylcholine- and serotonin-mediated airway contraction beyond PLC/PKC inhibition. (A) Concentration–response curve of rat PCLS stimulated with acetylcholine and pretreated with amitriptyline (1 µM). (B) Video-microscopic images of rats from (A). (C) Illustration of the part of the signal transduction cascade studied in Fig. 2. (D-H) Concentration–response curves of rat PCLS stimulated with acetylcholine (D, F–H) or serotonin (E) and pretreated with ipratropium bromide (1 µM) (D,E), U73122 (10 µM) (F,G) or chelerythrine (10 µM) (H) and amitriptyline (1 and 5 µM) or combinations. All graphs represent means ± SEM; *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. n = 4–5 in all groups. Up to three slices per condition were measured for each animal.
