Fig. 3 | Scientific Reports

Fig. 3

From: Amitriptyline inhibits bronchoconstriction independent of direct receptor binding and reduces number of caveolae

Fig. 3

Amitriptyline does not directly inhibit alternative pathways of bronchoconstriction. (A) Illustration of signaling pathways studied. (B,C) Concentration–response curves of rat PCLS stimulated with acetylcholine and pretreated with Y-27632 (10 µM) and amitriptyline (1 or 5 µM) or combinations. (D-G) Kinetic curves of rat PCLS stimulated with KCl (D) or serotonin (E–G) and pre-incubated with amitriptyline (0,1 µM, 1 µM, 5 µM) (D), or its combination with nifedipine (10 µM) (E–G). All graphs represent means ± SEM; * p ≤ 0.05, *** p ≤ 0.001. n = 3 in all groups. Up to three slices per condition were measured for each animal.

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