Fig. 1
From: Author Correction: Hypoxia mimetics restore bone biomineralisation in hyperglycaemic environments
Hypoxia (1% O2) and hyperglycaemia inhibited bone nodule formation. After 21 days, Alizarin Red stained (ARS) dense nodules were observed in (a) normal (5.5 mM) glucose, whilst (b) moderate (25 mM) and (c) high (50 mM) glucose conditions inhibited nodule formation. (d) Hypoxia normal glucose showed discrete biomineralisation that was not associated with collagen fibres. (e) Moderate and (f) high glucose inhibited biomineralisation. Transmission electron microscopy (TEM) micrographs of (g) normoxia normal glucose, (h) moderate glucose and (i) high glucose showed a glucose concentration dependent inhibition of bone nodule formation. COL fibres were not observed in (j) hypoxia normal glucose, but (k) moderate and high glucose environments in hypoxia showed some collagen fibres. Scale bar for (a–f) is 200 µm and for (g–i) is 2 µm (n = 5) (N: nodule, COL: collagen fibres, OB: osteoblast).
