Fig. 3
From: C-terminal fusion partner activity contributes to the oncogenic functions of YAP1::TFE3
Functional impact of TFE3 domains on the YAP and TFE3-related activity of YAP1::TFE3. (A) Schematics of the YAP1::TFE3 sequence and the different truncation mutants. (B) Representative YAP1-responsive 8xGTIIC-Luc reporter assay showing the YAP1 activity of wtYAP1, YAP1::TFE3, and wtTFE3 (n = 3 technical replicates). (C) Representative TFE3-responsive µE3-Luc reporter assay showing the TFE3 activity of wtYAP1, wtTFE3, and YAP1::TFE3 (n = 3 technical replicates). (D) Representative 8xGTIIC-Luc reporter assay showing the YAP activity of the different YAP1::TFE3 deletion mutants (n = 3 technical replicates). (E) Representative 8xGTIIC-Luc reporter assay showing the YAP activity of YAP1::TFE3 and S94A-YAP1::TFE3 (n = 3 technical replicates). (F,G) Representative µE3-Luc reporter assays showing the TFE3 activity of the different YAP1::TFE3 (F) and wtTFE3 (G) deletion mutants (n = 3 technical replicates). H) Representative µE3-Luc reporter assay showing the TFE3 activity of YAP1::TFE3 and S94A-YAP1::TFE3 (n = 3 technical replicates). Error bars indicate standard deviation. Analysis was done using ordinary one-way ANOVA. (*) P < 0.05; (**) P < 0.01; (****) P < 0.0001.
