Fig. 2 | Scientific Reports

Fig. 2

From: Transformation of Alternaria dauci demonstrates the involvement of two polyketide synthase genes in aldaulactone production and fungal pathogenicity

Fig. 2

Molecular characterization of A. dauci strains through PCR verification, visualized using 1.2% agarose gels. The individual gel photos are numbered 1 to 17 from top to bottom. Gel 1, amplification of ITS; gel 2, Hph; gel 3, AT domain of AdPKS7; gel 4, KS domain of AdPKS7; gel 5, AT domain of AdPKS8; gel 6, KS domain of AdPKS8; gel 7, NC region; gel 8, upstream region of AdPKS7ΔAT and downstream region of Hph; gel 9, downstream region of AdPKS7ΔAT and upstream region of Hph; gel 10, upstream region of AdPKS7ΔKS and downstream region of Hph; gel 11, downstream region of AdPKS7ΔKS and upstream region of Hph; gel 12, upstream region of AdPKS8ΔAT and downstream region of Hph; gel 13, downstream region of AdPKS8ΔAT and upstream region of Hph; gel 14, upstream region of AdPKS8ΔKS and downstream region of Hph; gel 15, downstream region of AdPKS8ΔKS and upstream region of Hph; gel 16, upstream region of ΔNC and downstream region of Hph; gel 17, downstream region of ΔNC and upstream region of Hph. Lane 1, wild-type A. dauci FRA001 strain; Lane 2, AdPKS7ΔAT; lane 3, AdPKS7ΔKS; lane 4, AdPKS8ΔAT; lane 5, AdPKS8ΔKS; lane 6, ΔNC; lane 7, negative control. While the gels in this figure are cropped, the individual, whole gel photos are provided in Supplementary document 1.

Back to article page