Table 1 First cytotoxic doses (IC10) of conventional liposomes (L) and pegylated liposomes (PegL) encapsulated with free stigmasterol (St), stigmasterol mirystate (ME), and stigmasterol oleate (OE), both undigested and gastrointestinally digested (GID), determined in normal human small intestinal HIEC-6 and colon mucosa CCD 841CoN cell cultures.

From: Effect of gastrointestinal digestion on the stability and cytotoxicity of conventional and pegylated liposomes encapsulated with stigmasterol and its esters

Liposomes

Cytotoxic dose (IC10) (µg/ml)

HIEC-6 cells

CCD 841CoN cells

L-St

> 100

95.00 ± 10.77

GID L-St

25.40 ± 3.94***

3.02 ± 0.48***

PegL-St

3.01 ± 0.43###

3.38 ± 0.79###

GID PegL-St

9.28 ± 0.89***

2.80 ± 1.13

L-ME

1.06 ± 0.13

0.31 ± 0.05

GID L-ME

6.30 ± 1.38**

1.57 ± 0.52**

PegL-ME

0.82 ± 0.10

0.22 ± 0.03

GID PegL-ME

13.21 ± 4.97**

2.42 ± 0.47*

L-OE

2.51 ± 0.11

3.84 ± 0.59

GID L-OE

25.39 ± 3.12***

8.40 ± 1.42**

PegL-OE

3.56 ± 1.11

4.07 ± 0.55

GID PegL-OE

14.26 ± 6.66**

1.66 ± 0.21*

  1. Values represent the means (n = 3) ± SD. Significant differences: *digested liposomes compared to undigested liposomes (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001); #PEGylated liposomes compared to non-PEGylated liposomes (#p ≤ 0.05; ##p ≤ 0.01; ###p ≤ 0.001).
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