Fig. 6
From: Protocol optimization improves the performance of multiplexed RNA imaging
Optimized Protocols Improve MERFISH Performance in Tissue Samples. (A, B) The spatial distribution of 7 out of ~ 1000 mRNAs profiled in a Swiss roll of the mouse colon using a published library and published protocols (A) or using a new library that removes high-background readout probes and the optimized protocols presented here (B). Zoom-ins are boxed. (C) mRNA abundance determined with the new library and optimized protocols as in (B) versus that measured with the published library and protocols in (A). mRNAs are colored by readout position score (Methods). (D) Distribution of the abundance of blank barcodes measured in fraction of all detected barcodes. (A, B). (E) Spatial distribution of Foxp3 (top) or Foxl1 (bottom) in the measurement in (A; left) or (B; right). All mRNAs are plotted in gray and these two mRNAs are plotted in red. For A, B: Scale bars: 1 mm.
