Fig. 2 | Scientific Reports

Fig. 2

From: High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration

Fig. 2

Integrated FROS reduces switching efficiency. (A) Example image and schematic illustration of two FROSs labeling the mating-type switching loci, with ~ 182kbp genomic distance, in live yeast cells. Homology regions for integration are specified in parentheses. (B) The effect of FROS genome editing on mating type switching efficiency in the different strains that are illustrated and described below each bar plot. The frequency of switching is significantly influenced both by genomic perturbations next to the mating-type switching related loci, and by the diffusing fluorescence repressors (p-value < < 0.01; t-test). Abbreviations: I & R - Inducible and reporting strains containing the HO-CFP plasmid; dif - diffuses in the nucleus; loc – localized in specific locus (HMLα or MAT). Fluorescence images of the strains are presented in figure S4-B.

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