Fig. 2

Lipid sorting and formation of lipid nanodomains. (A) Three-dimensional deformation of a planar membrane with the surface map for sorting of eight different lipid classes with varying bending moduli. Crosslinkers are not shown. Left: Angled view. Right: Planar view. (B) Lipid number strain α profile for the membrane in (A). Crosslinkers are shown here. (C‒J) The pattern of lipid nanodomains, shown separately for each lipid class. (K‒N) Expanded views of patterns in (D‒G). (O) Plots of the mean and standard deviation (SD) of the area of the nanodomains. Mean values are 20,183 \({\text{nm}}^{2}\) (\({10k}_\mathrm{b}T\), SD: 11 \({\text{nm}}^{2}\), n: 9), 19,785 \({\text{nm}}^{2}\) (15.7\({k}_\mathrm{b}T\), SD: 39 \({\text{nm}}^{2}\), n: 9), 12,897 \({\text{nm}}^{2}\) (21.4\({k}_\mathrm{b}T\), SD: 8476 \({\text{nm}}^{2}\), n: 13), 1786 \({\text{nm}}^{2}\) (27.1\({k}_\mathrm{b}T\), SD: 1975 \({\text{nm}}^{2}\), n: 146), 1209 \({\text{nm}}^{2}\) (32.9\({k}_\mathrm{b}T\), SD: 1117 \({\text{nm}}^{2}\), n: 222), 1060 \({\text{nm}}^{2}\) (38.6\({k}_\mathrm{b}T\), SD: 1194 \({\text{nm}}^{2}\), n: 262), 1480 \({\text{nm}}^{2}\) (44.3\({k}_\mathrm{b}T\), SD: 1886 \({\text{nm}}^{2}\), n: 194), and 8571 \({\text{nm}}^{2}\) (\({50k}_\mathrm{b}T\), SD: 9642 \({\text{nm}}^{2}\), n: 35). Individual domain areas are indicated by colored dots. Blue: Multi-element domain. Red: Single-element domain. See Fig. S14 for the degrees of freedom of each element. See Movie S1 for all membrane-crosslinker configurations used in the analyses in this figure.