Fig. 4
The effect of the number and distribution of crosslinkers and lipid composition on nanomechanical responses of membrane-crosslinker complexes. (A) The deformed configuration with the surface strain map for a \(1.944\times 1.944 \; \mathrm{\mu {m}^{2}}\) membrane (\({\kappa}=20{k}_\mathrm{b}T\)) and five crosslinkers when the center crosslinker (\(c=3\)) was pulled with a 249 nm displacement (left). An expanded view of the membrane-crosslinker complex with the surface map for the sum of normalized \({M}_{c}\) values (right). (B) A deformed configuration with the surface strain map for the membrane-crosslinker complex in (A) by considering 75% \(50{k}_\mathrm{b}T\) lipids and 25% \(20{k}_\mathrm{b}T\) lipids. The corresponding lipid sorting map is shown on the right. (C) The force vs. displacement of the red bead at the center (\(c=3\)) in (A) is shown with blue circles, and compared to experimental data11. The force vs. displacement for the center red bead in (B) is shown with red diamonds. (D) The deformed configuration with the surface strain map for the \(1.944\times 1.944 \; \mathrm{\mu {m}^{2}}\) membrane (\({\kappa}=20{k}_\mathrm{b}T\)) and twenty-one crosslinkers when the center crosslinker (\(c=11\)) was pulled with a 281 nm displacement (left). An expanded view of the membrane-crosslinker complex with the surface map for the sum of normalized \({M}_{c}\) values (right). (E) A deformed configuration with the surface strain map for the membrane-crosslinker complex in (D) by considering 75% \(50{k}_\mathrm{b}T\) lipids and 25% \(20{k}_\mathrm{b}T\) lipids. The corresponding lipid sorting map is shown on the right. (F) The force vs. displacement of the red bead at the center (\(c=11\)) in (D) is shown with blue circles and compared to experimental data11. The force vs. displacement for the center red bead in (E) is shown with red diamonds. See Figs. S16 and S17 for the degrees of freedom. Movies S4‒S7 visualize all membrane-crosslinker complexes used in the analyses in this figure.
