Fig. 5

Edited clones successfully differentiate into RPE cells and retain functional kill switch. (a) Overview of differentiation protocol from iPSCs into RPE cells. (b-c) Flow cytometry quantification of of PMEL17 (b) and TYRP (c) expression. (d) RT-qPCR quantification of RPE-specific mRNAs. Expression of edited cells is normalized to expression of parental line (e–f) RT-qPCR quantification of iCASP9 expression (e) and B2M levels (f). Expression of edited cells is normalized to expression of parental line. (g-h) Brightfield images (5 × objective) of parental cell lines and edited cells before killing (g) and after recovery (h). Scale bars = 100 µm.