Fig. 4
Astronauts experience early, late and long-term changes in H3K27ac in CD8+ immune cells upon prolonged orbital spaceflight. (a–f) Top: Boxplot showing changes in H3K27ac in CD8+ immune cells following prolonged orbital spaceflight. Shown are clusters with loss (a-c) or gain (d-f) in H3K27ac that show an early response/change in R+1 (a, d), late response/change in R + 35 (b, e) or long-term response/change in R+1 and R + 35 (c, f). H3K27ac signal of astronauts and control subjects pre-flight (PF) and post-flight at R+1 and R+35 ±15 was retrieved from bigwig files. The RPKM signal of the differential regions was averaged over each replicate subject and is displayed relative to the median of the PF sample in each group (astronaut or control). Box plot with 2.5 and 97.5 percentiles with individual data points shown only for values falling outside this interval, statistical analysis: Friedman test and Dunn’s multiple comparisons test (****: p ≤ 0.0001). Combined brackets signify same significance for all three comparisons (PF vs R+1 vs R + 35). Bottom: Representative Cut&Tag tracks of the boxplot of RPKM normalised H3K27ac intensity in CD8+ immune cells of control subjects and astronauts pre-flight and post-flight at R+1 and R+35 ±15. All samples were grouped to the same maximum signal indicated in the first line. Each replicate is shown in a different shade of green and overlaid on the same track. n = 3.
