Fig. 4 | Scientific Reports

Fig. 4

From: Investigating urea is an ideal additive to the Accu-OptiClearing delipidation cocktail

Fig. 4

Application of the modified clearing solution in neural tracing. (A) Orthogonal projection of the AAV9-DIO-EYFP and the AAV2-retro-Cre-mCherry signals in the Antero-System, labeling the VTA-CA1 neural circuit (z-depth: 347.65 μm). (B) Color-coded projection of the anterograde AAV9-DIO-EYFP signals connecting VTA to CA1 in the same brain section (z-depth: 347.65 μm). VTA, ventral tegmental area; CA1, Cornu Ammonis 1.

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