Fig. 2

The dendritic trees of principal neurons show spatial restriction to the subnucleus where the soma is located. (a) Experimental setup. In vitro whole-cell recording and labeling of PNs in coronal and horizontal slice preparations, focusing on distinct subnuclei of LA and BA. (b) Confocal images of biocytin filled example neurons showing restricted dendritic trees to the LA and BA subnuclei. Images of example PNs taken from coronal (LAp PN and BAp PN) and horizontal (LAa PN and BAa PN) slices. Scale bar: 100 μm. (c) Reconstructed dendritic trees of 14 representative PNs from LA and BA subnuclei obtained from 6 different coronal sections along the anteroposterior axis of the amygdala. Scale bar: 500 μm. (d) Bar graph showing the proportion of dendritic trees located within and outside of DsRed + areas in coronal slices (LAa: n = 9 (red); LAp: n = 8 (light gray); BAa: n = 9 (dark red) and BAp: n = 9 (dark gray); 35 PNs from 17 mice). Dendritic trees of PNs with somata located in the LAa and BAa are restricted to DsRed + areas. Conversely, dendritic trees of PNs with somata located in the LAp or BAp are restricted to DsRed- areas. (e) Reconstructed dendritic trees of 15 representative PNs from the LA and BA subnuclei obtained from 7 different horizontal sections along the dorsoventral axis of the amygdala. (f) Bar graph showing the proportion of dendritic trees located within and outside of the DsRed + areas in horizontal slices (LAa: n = 8 (red); LA: n = 13 (light gray); BAa: n = 9 (dark red) and BAp: n = 23 (dark gray); 53 PNs from 23 mice). Dendritic trees of PNs with somata located in the LAa and BAa are restricted to DsRed + areas. Conversely, dendritic trees of PNs with somata located in the LAp or BAp are restricted to DsRed- areas. Scale bar: 500 μm.