Table 3 PCR reaction system.

From: LncRNA HOXA11-AS promotes the cell proliferation, migration, invasion and inhibits cell apoptosis in esophageal squamous cell carcinoma

Component

Added amount (µl)

2X SYBR Green Pro Taq HS Premix(ROX plus)

10

Primer F(10 µM)

0.4

Primer R(10 µM)

0.4

cDNA

2

RNase free water

7.2