Fig. 1
From: Efficient transgenic maize (Zea Mays L.) detection using low-depth next-generation sequencing
Detection of transgenic nucleic acid sequences in maize using low-depth sequencing. A Schematic of transgenic maize nucleic acid sequences, highlighting the LB and RB flanking the exogenous gene insertion site. B The quantity of detected reads across varying read length thresholds (5–50 bp, 5 bp increments). The 20 bp threshold matched empirical detection results (ND207_total reads_reliability). C, D IGV validation of ND207-specific reads at 20 bp alignment: C LB junction (4 reads); D RB junction (6 reads). E, F Empirical confirmation of detected reads: (E) LB (4 reads); (F) RB (6 reads). Data represent mean ± SD of ten biological replicates. Lowercase letters denote statistically significant differences between groups (one-way ANOVA, P < 0.05).
