Fig. 3
From: Efficient transgenic maize (Zea Mays L.) detection using low-depth next-generation sequencing
Impact of DNA extraction methods on transgenic sequence detection in maize using NGS. A Quantification of ND207-specific reads aligned to LB and RB sequences using four DNA extraction methods: The Maxi Kit yielded the highest read counts. B–I IGV validation of ND207-specific reads. CTAB: (B) LB (2 reads), (C) RB (4 reads). DNeasy Maxi Kit: (D) LB (7 reads), (E) RB (7 reads). DNeasy Mini Kit: (F) LB (5 reads), (G) RB (6 reads). Plant Genomic DNA Kit: (H) LB (3 reads), (I) RB (2 reads). Data represent mean ± SD of ten biological replicates. Lowercase letters denote statistically significant differences between groups (one-way ANOVA, P < 0.05).
