Fig. 4
From: Efficient transgenic maize (Zea Mays L.) detection using low-depth next-generation sequencing
The impact of different library construction methods on the detection of ND207 transformants. A Total reads generated by PCR-Free, Tn5 digestion, mechanical shearing, and enzymatic digestion methods. B Detection efficiency of ND207-specific sequences across library methods. C–J IGV validation of ND207-specific reads. PCR-Free: (C) LB (8 reads), (D) RB (6 reads). Tn5 digestion: (E) LB (5 reads), (F) RB (4 reads). Mechanical shearing: (G) LB (5 reads), (H) RB (6 reads). Enzymatic digestion: (I) LB (5 reads), (J) RB (5 reads). Data represent mean ± SD of ten biological replicates. Lowercase letters denote statistically significant differences between groups (one-way ANOVA, P < 0.05).
