Fig. 1
From: Assessing the potential of bacterial signal peptides for radiopharmaceutical applications
Characterization of formylated peptide ligands for FPR receptor specificity, binding affinity, and calcium mobilization. (a) Heatmap displaying the EC50 values for peak calcium responses determined through the signal amplitude (dF/F0) of FPR1, FPR2 and FPR3 transfected HEK293T cells after stimulation with different peptide derivatives. Peptide sequences are displayed in the one letter code, f- denotes the formyl group at the N-terminus. (b) Concentration-dependent activation of Ca2+ mobilization by f-MVPIKI in FPR1 transfected HEK293T cells. Error bars denote SD, n = 6; N = 6. c: Upper panel: representative microscopic images displaying the concentration dependent receptor affinity of the fluorescent labelled f-MVPIK(FITC)I probe bound to FPR1, FPR2, FPR3 and mock transfected HEK293T cells. Lower panel: concentration dependent binding of two different fluorescence labelled peptides f-MVPIK(FITC)I and f-MVPIK(TAMRA)I to HEK293T cells transfected with mock, FPR1, FPR2 or FPR3, respectively. Error bars denote SD, n = 3; N = 9. d: Calcium response of FPR1 transfected HEK293T cells upon stimulation with 1 nM f-MVPIKI, f-MVPIK(FITC)I or f-MVPIK(TAMRA)I display similar sensitivity. Error bars denote SD, n = 3; N = 6.
