Fig. 5
From: Assessing the potential of bacterial signal peptides for radiopharmaceutical applications
Metabolic stability and cellular internalization of radiolabeled peptide. (a) Representative UV/vis-RP-HPLC chromatograms of the bioconjugate f-MVPIK(Ahx-DOTA)I and its natGa- and natLu complexes. (b) Representative radio-RP-HPLC chromatograms of [68Ga]Ga-f-MVPIK(Ahx-DOTA)I and [177Lu]Lu-f-MVPIK(Ahx-DOTA)I. (c) Stability of [68Ga]Ga-f-MVPIK(Ahx-DOTA)I and [177Lu]Lu-f-MVPIK(Ahx-DOTA)I in human plasma assessed by radio-RP-HPLC, normalized. (d) Cellular internalization of [177Lu]Lu-f-MVPIK(Ahx-DOTA)I in transfected HEK293T cells over 24 h, total counts (in counts per minute, cpm) and percentage of applied activity. (e) Internalization of [177Lu]Lu-f-MVPIK(Ahx-DOTA)I in U87-MG cells over 24 h, total counts (cpm) and percentage of applied activity. The striped columns represent blocking experiments in the presence of the specific FPR1 antagonist BOC-FLFLF.
