Fig. 1 | Scientific Reports

Fig. 1

From: Development and characterization of a human papillomavirus-based nanoparticle carrier for heterologous vaccine antigens

Fig. 1

Preparation and characterization of L1-VLPs. (A) Analysis of the expression and purification of HPV-16 L1 protein, visualized by SDS-PAGE and Coomassie blue staining. Lane 1 shows the sample without IPTG induction during E. coli expression, while Lane 2 shows the sample with IPTG induction. Lanes 3–5 represent the sequential purification steps, including samples obtained after ammonium sulfate precipitation, cation-exchange chromatography and SEC. (B) The in vitro disassembly-reassembly process was monitored using SEC. (C) TEM images of purified L1 aggregates, L1 pentamers and L1-VLPs.

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