Fig. 5
Env- and L1-specific IgG serum concentrations after immunization with L1-Env. Mice were vaccinated at week 0 and 4 with 60 µL of either low, medium, or high doses of L1-Env nanoparticles or corresponding low, medium, or high doses of L1-VLPs mixed with soluble Env as controls (see Table 1 for antigen amounts). Blood samples were drawn at week 3 and 6. (A) The levels of Env-specific IgG antibodies in the sera were evaluated using a serum ELISA, with results expressed as relative light units per second (RLU/s). Data are presented as the mean ± standard error of the mean (SEM) for n = 4. Statistics were performed using Mann-Whitney tests. (B) The levels of L1-specific IgG antibodies in the sera (expressed as RLU/s) are shown, with each column representing the mean ± SEM of the respective group. Statistical differences between groups were assessed using the Mann-Whitney test. All significance levels are indicated as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
