Table 2 Densitometric analysis of SDS-protein PAGE for leaf of six soybean varieties through three planting dates infested by soybean stem flies.

From: Impact of planting dates on yield and resistance of soybean varieties to soybean stem fly (Melanagromyza sojae) in Egypt

MW (KDa)

Mid-May

Beginning of June

Mid-June

 

1

2

3

4

5

6

1

2

3

4

5

6

1

2

3

4

5

6

400

350

 +1

 +1

 +1

 +1

 +1

 +1

 +1

 +1

 +1

 +1

 +1

300

 + 1

 +1

 +1

240

 +1

 +2

 +3

 +4

 +3

 +3

 +4

 +4

 +4

 +4

 +4

 +4

 +3

 +3

210

 +2

 +3

180

 +4

 +4

 +4

 +3

 +3

 +3

 +4

 +3

 +3

 +3

 +3

 +3

 +4

 +3

 + 3

 +3

 +3

 +3

150

 +2

 +2

 +2

 +2

 +2

 +2

 +2

100

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

90

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

 +2

75

 +4

 +4

 +4

 +4

 +4

 +4

 + 4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

65

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

55

 +4

40

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

 +4

20

Total

10

10

9

8

7

7

9

9

8

7

8

8

8

7

7

6

6

9

  1.  + 4: v.v. dark, + 3: dark, + 2: Clear, + 1: Faint, –: absent, MW = Molecular weight, M = Marker, 1 = Giza 21, 2 = Giza 22, 3 = Giza 35, 4 = Giza 111, 5 = Crawford, 6 = Dr-101. The densitometric analysis of SDS-protein PAGE for the leaf sample showed a very dark band at position + 4, indicating a high concentration of the protein of interest. This suggests that the leaf sample has a significant amount of the target protein compared to other bands on the gel. In contrast, there was an absence of any band at position -, suggesting that this protein was not present in the sample.