Fig. 5 | Scientific Reports

Fig. 5

From: PD-L1 autoregulation promotes the proliferation, migration and invasion of glioblastoma cells via GP130/JAK2/STAT3/IRAK2/IL6 signaling pathway

Fig. 5The alternative text for this image may have been generated using AI.

STAT3 regulated IRAK2/NFκB/IL6 signaling pathway. (A) Correlation of STAT3 expression and IRAK2 expression in TCGA-GBM dataset in cbioportal. (B) STAT3 binding of IRAK2 promoter. JASPAR was used to predict potential binding sites. (C) Dual luciferase reporter assay showing that STAT3 was bound to IRAK2 promoter. (D) Effect of silencing STAT3 on IRAK2 protein expression. Cells were transfected with siNC or siSTAT3. Protein expression levels of STAT3 and IRAK2 were detected by Western blot. (E) Dual luciferase reporter assay showing that STAT3 was bound to PD-L1 promoter. (F) Effect of silencing IRAK2 on P-P65, P65 and IL6 expressions. (G–K) Effect of silencing IRAK2 on cell viability (G), colony formation (H), proliferation (I), migration and invasion (J) as well as wound healing capacity (K). *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: No statistically significant.

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