Fig. 1
From: EHE cell cultures are a platform for mechanistic and therapeutic investigation
Derivation of EHE primary cell cultures and demonstration of the TAZ-CAMTA1 fusion protein (WWTR1-CAMTA1 fusion gene). (A) Table containing patient characteristics associated with EHE tumors. (B) H&E staining and immunohistochemical evaluation of CAMTA1 expression in EHE 036 and EHE 053. Images taken at 200X. (C) Schematic outlining generation and characterization of the extended primary cell cultures. (D) Image of primary cell cultures in a monolayer with insets at higher power. Arrow denotes tumor fragment of EHE 036. (E) Schematic representation of RNA Breakpoint for EHE 001 determined by RT-PCR. Codons with their associated amino acids are shown below. (F) Schematic representation of RNA Breakpoint for EHE 036 by anchored multiplex PCR from tumor RNA. Codons with their associated amino acid are shown below. (G) qRT-PCR utilizing custom probes hybridizing to the EHE 001 and EHE 036 breakpoints respectively, with HPAEC as a control. (H) Single-cell RNA sequencing of early passage EHE 001 (passage number 6) demonstrating expression of CAMTA1. (I) Single-cell RNA sequencing of early passage EHE 001 (passage number 6) demonstrating expression of FLI1. (J) Western blot, after enriching for the nuclear fraction, probing for TAZ in HPAEC control and the EHE extended primary cell cultures. Error bars were used to define one standard deviation. For all panels, ****p < 0.0001, *** p < 0.001, ** p < 0.01, *p < 0.05.
