Fig. 5
From: EHE cell cultures are a platform for mechanistic and therapeutic investigation
Composition of early and late passage EHE extended primary cell cultures. (A) Plot showing the growth rate of the EHE extended primary cell cultures as a function of days passaged. Data points on the line graphs represent passages. Human pulmonary artery endothelial cells (HPAECs) are included as a control. (B) t-distributed stochastic neighbor embedded (t-SNE) plot showing unbiased clustering of EHE 001 cells by Loupe browser. (C) GO Biological Process pathway enrichment analysis for Cluster (1) (D) GO Biological Process pathway enrichment analysis for Cluster (2) (E) GO Biological Process pathway enrichment analysis for Cluster (3) (F) GO Biological Process pathway enrichment analysis (top) and Kyoto Encyclopedia of Genes and Genomes (KEGG) (bottom) analysis for Cluster (4) Identical thresholding for relative gene expression maintained for early and late passaging and was also maintained for the comparisons in (G-K). (G) Comparison of number of PCNA positive cells (purple) between P6 and P17. (H) Comparison of CD44 positive cells between P6 and P17. (I) Comparison of CDKN2A positive cells normalized by transcriptional activity (UMI/cell count) between P6 and P17. (J) Comparison of TP53 positive cells normalized by transcriptional activity (UMI/cell count) between P6 and P17. (K) Comparison of MT-CO3 positive cells normalized by transcriptional activity (UMI/cell count) between P6 and P17. (L) Working model for different cell subsets in EHE extended primary cell cultures.
