Fig. 1 | Scientific Reports

Fig. 1

From: The free-living ciliate Tetrahymena pyriformis inactivates engulfed influenza A(H1N1)pdm09 virus via two distinct types of endosomes

Fig. 1

Quantification of A(H1N1)pdm09 RNA in cell lysates and culture supernatants obtained from the A(H1N1)pdm09/ T. pyriformis co-culture. The A(H1N1)pdm09 virus and T. pyriformis were co-cultivated at 25°C and 0.5 ml samples were collected at specified time points. Reverse transcription followed by Q-PCR was conducted on viral RNA isolated from T. pyriformis cell lysates (white) and culture supernatants (black). The unmixed A(H1N1)pdm09 culture was used as a control (grey). The mean virus concentration was genome equivalent per ml (GE/ml) and Standard Deviation (SD) from 3 independent experiments are shown. The detectable limit was 1000 GE/ml; p<0.05 compared to the1.5 h time point.

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