Fig. 4

Temporal and spatial distribution of A(H1N1)pdm09 captured by T. pyriformis cells. The A(H1N1)pdm09 virus and T. pyriformis were co-cultivated at 25°C; at specified time points 100 μl samples were transferred to wells of the 96-well plates/glass slides, fixed with paraformaldehyde and A(H1N1)pdm09 was visualized using nucleoprotein -specific monoclonal antibodies (mabs NP). The images were taken with fluorescent (a) and CLSM (b) microscopy. A comparison is provided as a control the staining of control protozoan cells with those treated with the mabs to NP.