Fig. 1
Experimental Design. Female Swiss Webster mice were habituated to the equipment by exposure to room air for 48 or 96 min/day for 5 days. Next, they were acclimated to their exposure conditions (room air, e-liquid or e-cigarette; n = 8 per group) daily for 2 weeks. Then, the female mice were bred with unexposed GAD67-GFP+/− heterozygous male mice. Daily exposure of the mice continued through pregnancy until gestational day 14 (GD14; day of conception was designated as GD0). Trunk blood from e-cigarette exposed dams and brains from their embryos were collected on GD14 for cotinine analysis. Brains from GAD67-GFP+/− embryos from each of the three exposure groups were collected on GD15 (24 h. after the final room air or aerosol exposure), for analysis of GABA neuron migration in histological sections of the forebrain.
