Fig. 2
GABA Cell Density in the E15 Dorsal Forebrain. Representative coronal sections at the rostral (A) and caudal (E) levels of the forebrain from a GAD67-GFP+/− embryo showing GFP + GABA neurons (green). DAPI (blue) was used to label cell nuclei. GABA neurons were counted within a region of interest (white dashed rectangles) at each of three anatomical locations along the lateral to medial extent of the dorsal cerebral wall: lateral (a), middle (b), and medial (c). A higher magnification view of the “middle” anatomical location (b) of the dorsal cerebral wall from the rostral (B) and caudal (F) levels showing subdivision into 10 equal-sized grids for the purpose of counting GFP + GABA neurons, and stratification into marginal zone (MZ), cortical plate (CP), intermediate zone (IZ), and subventricular/ventricular zones (SVZ/VZ) (B, F). GABA neuron density is reduced in the e-cigarette group compared to the room air group at the rostral level (C) and there is no significant difference among the 3 groups at the caudal level (G). GABA neuron density was reduced in the e-cigarette group compared to the room air group in the MZ at the rostral level (D) while there were no significant differences among the groups in any lamina at the caudal level (H). Scale bars represent 300 µm (A, E) and 20 µm (B, F). * = p < 0.05 [Pair-wise comparisons with Dunnett’s (C) or Benjamini–Hochberg linear step up (D) corrections]. Data represent mean ± SEM.
