Fig. 6 | Scientific Reports

Fig. 6

From: Total flavonoids of rhizoma drynariae improved the osteogenic and pro-angiogenic capacities of BMSCs to accelerate long bone regeneration

Fig. 6

BMSCs-CM from TFRD-treated mice improved HIF-1α expression by inhibiting YAP1/TAZ in ECs under hypoxic conditions. (A) The expression levels of the YAP1/TZA target genes CTGF and CYR61 and the HIF-1α target gene VEGFA in HUVECs induced with BMSC-CM from TFRD-treated mice compared with those in control mice under hypoxic conditions (1% O2) (n = 6). Immunofluorescence images and quantitative analysis of (B) YAP1, (C) TAZ and (D) HIF-1α in HUVECs induced with BMSC-CM from TFRD-treated or saline-treated mice under hypoxic conditions; scale bar = 100 μm. (E) Immunofluorescence images of HIF-1α in HUVECs from the si-ctrl, si-YAP1, si-TAZ, si-YAP/TAZ and si-YAP/TAZ + TFRD groups (n = 6); scale bar = 100 μm. (F) Quantitative analysis of the mean intensity of HIF-1α in HUVECs from each group under hypoxic conditions (n = 6).

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