Fig. 2
In 2A, the characteristics of CH4 production at varying pressures using the MM-CF chloride medium. Reaction sets 1–6 correspond to cultivations at 3 bar (n = 4), and sets 7–9 correspond to cultivations at 10 bar (n = 2). For each reaction set, the methane evolution rate (MER) and specific methane production rate (\(q_{{{\text{CH}}_{{4}} }}\)) are illustrated as the average of the maximum MER values across all reactors. Colored diamonds represent individual reactor values. Error bars indicate the standard deviation of the mean values, showing variability in CH4 production across replicates. In 2B, biomass growth characteristics at different pressures using the MM-CF chloride medium are shown. Reaction sets 1–4 represent biomass cultivation at 3 bar (n = 4), while reaction sets 5–7 are cultivated at 10 bar (n = 2). Optical density (OD578) values are shown as mean values across all reactors. Colored rectangular markers represent OD578 measurements before each reaction cycle set, and triangular markers represent OD578 measurements after each cycle set, capturing biomass growth over time. × -markers between reaction set 2/3 and 5/6 indicate linear interpolated values. No measured data is available, as reaction sets were performed sequentially without sampling. Colored diamonds indicate individual OD578 values for each reactor, contributing to the mean. Hatched rectangles illustrate the net change in OD578, indicating biomass growth across cycles. Error bars represent standard deviations, showing variability across replicates.
