Table 2 The primary antibodies used for immunofluorescence staining.
From: Ptbp1 knockdown induces conversion of rat spinal cord astrocytes into neuron like cells
Antibodies | Species | Dilution | Company | Catalog | RRID |
|---|---|---|---|---|---|
beta III tubulin | Rabbit | 1:1000 | Abcam | ab52623 | AB_869991 |
NeuN | Rabbit | 1:500 | Abcam | ab177487 | AB_2532109 |
MAP2 | Rabbit | 1:500 | Abcam | ab183180 | AB_2895301 |
MAP2 | Chicken | 1:200 | Abcam | ab318993 | Â |
ChAT | Sheep | 1:200 | Abcam | ab18736 | AB_2244867 |
Nestin | Rabbit | 1:300 | Abcam | ab316018 | Â |
GFAP | Mouse | 1:1000 | Proteintech | 60,190–1-Ig | AB_10838694 |
S100β | Rabbit | 1:250 | Abcam | ab52642 | AB_882426 |
Ptbp1 | Mouse | 1:500 | Proteintech | 67,462–1-Ig | AB_2882692 |
SOX9 | Mouse | 1:1000 | eBioscience | 14–9765-82 | AB_2573006 |
