Fig. 2
SOD1G93A zebrafish exhibit decreased type 1 and type 2 myofiber cross-sectional area in the skeletal musculature. Bright field images of wildtype and SOD1G93A zebrafish, and silhouettes of these images (black) at (A) 20 weeks of age, and (B) 40 weeks of age. The red line denotes the thickness of the trunk. (C) Animal weight and (D) thickness of their trunk measured at 20 and 40 weeks of age (n = 25). (E) Image of a hematoxylin and eosin stained tissue section obtained from a zebrafish severed at the anal fin. Black bar = 500 µM. (F) Drawing depicting the location of Type 1 and Type 2 fibers in the trunk lateral and caudal musculature. Hematoxylin and eosin stained tissue sections from the truck caudal musculature of (G,I) wildtype and (H,J) SOD1G93A zebrafish at the 40-week time point. Black bar = 50 or 100 µM, as indicated. (K) Graph of the mean cross-sectional area of Type 2 fibers in the caudal musculature of SOD1G93A and wildtype zebrafish at the 40-week time point (n = 18–20). (L) Distribution of Type 2 fiber cross-sectional areas in SOD1G93A and wildtype zebrafish at the 40-week time point. Hematoxylin and eosin stained tissue sections of Type 1 fibers in the lateral musculature of (M) wildtype and (N) SOD1G93A zebrafish at the 40-week time point. Black bar = 50 µM. (O) Graph of the mean cross-sectional area of Type 1 fibers in SOD1G93A and wildtype zebrafish at the 40-week time point (n = 18–20). (P) Distribution of type 1 fiber cross-sectional areas in SOD1G93A and wildtype zebrafish at the 40-week time point. * = p ≤ 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001.
