Fig. 4

Analysis of eWAT in HFD-fed WT and P7KO mice. (A) Western blotting and (B) quantitative analysis of PARIS expression in eWAT of WT (W; gray bars) mice fed with ND or HFD. Data presented as means ± SD, analyzed by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test; ****P < 0.0001. (C) Representative microscopic images of H&E-stained eWAT of WT and P7KO (KO) mice. Scale bars: 200 µm. (D) Distribution curve and box plot of adipocyte size in the two groups: WT, n = 814 cells from four mice; KO, n = 1,143 cells from four mice. (E) RT-PCR analysis of mRNA expression of Il6 and Il10 in eWAT of WT (gray bars) and P7KO (red bars) mice. (F) Western blotting and (G) quantitative analysis of PPARγ isoforms 1 and 2 in eWAT of WT and P7KO mice (n = 4). Ponceau-S staining was used for normalization. (H) RT-PCR analysis of mRNA expression of Pparg1, Pparg2, Adipoq, Fasn, Acaca, Srebf1, Cebpa, and Ppargc1a in eWAT of WT and P7KO mice. Data expressed as means ± SD, analyzed by Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001.