Fig. 1

Single-cell RNA-Seq analysis of the thyroid gland. (a) Workflow of sample collection, scRNA-seq, and data analysis. (b) UMAP plot showing cell clustering and annotation across all samples. (c) UMAP plot color-coded by individual sample identifiers. (d) Bubble plot showing the expression of canonical marker genes across identified cell types. (e) Bar plot of cell type composition across different groups. (f) Manhattan map of DEGs among clusters, highlighting transcriptional differences across different groups.