Fig. 6 | Scientific Reports

Fig. 6

From: Robinin attenuates cardiac oxidative stress-induced endoplasmic reticulum-dependent apoptosis through AKT/GSK3β pathway

Fig. 6

(A) Triphenyl Tetrazolium Chloride stained transverse slices of the heart of Control, ISO treated, Rob and Rob + ISO treated groups. TTC stains viable cardiac muscle deeply red, whereas regions of infarction are pale. Photomicrograph of H&E stained sections of left ventricular tissues, (B) Quantitative analysis of infarct size. Infarct size was calculated by the ratio of the infarction area to the total left ventricular section area and quantified using ImageJ software. (C) Cardiomyocytes shows regular striated structure in control group. (D) In ISO group cardiomyocytes shows altered morphology, the nuclei are irregular in shape, the cytoplasm is devoid of organelles (star), damaged myocytes presented with loss of cellular organization, diffused/focal inflammatory infiltrate ( ). (E) Rob alone group shows morphology similar to that of control. (F) In Rob + ISO group heart tissue with improved structural and morphological features similar to healthy control. Scale bar: 100 µm. Data are presented as mean ± SD of six independent experiments. SD, standard deviation.

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