Fig. 6
RNA secondary structure prediction analysis and relative NrdA protein levels for nrdAB with full-length and truncated 5’ UTR variants. (a) Table summarizing minimum free energy (MFE) values for predicted secondary structures for nrdAB mRNA with full 5’ UTR (nrdAB) and with truncated 5’ UTR (nrdAB- Δ5’ UTR), along with free energy values for ribosome binding site (RBS) accessibility, calculated using RNAfold software50. (b) Mountain plots illustrating the predicted optimal secondary structures. The x-axis indicates the position of each base pair (Sequence, bp) and the y-axis (Height) represents the cumulative number of base pairs enclosing each position. Blue and green arrows mark the 5’ and 3’ ends of the mRNAs, respectively. (c) Western blot analysis of relative NrdA protein levels in P. aeruginosa PAO1 WT and PAO1 Δ5’ UTR (nrdA) strains during exponential (EX) and stationary (ST) phases under both aerobic and anaerobic conditions. Membranes were stained with Ponceau S prior to antibody incubation to confirm equal protein loading (Supplementary Material Figure S3). The original and cropped version of the gel are provided in the Supplementary Material Figure S6 and are also available in the public repository (https://doi.org/10.34810/data2361).
