Fig. 1 | Scientific Reports

Fig. 1

From: Whole-genome nanopore sequencing and automatic downstream analysis of respiratory syncytial virus using RSVTyper

Fig. 1

Overview of the RSVTyper pipeline. (A) Wetlab workflow. First, RNA was extracted and transcribed into cDNA, which was then amplified in a multiplex tiling PCR in two primer pools. The PCR products were pooled and libraries were prepared for nanopore sequencing. (B, C) RSV cell culture isolates and their G clades are depicted for each sampling year, spanning from 2008 to 2018. (D) Genome coverage of each sample is depicted in relation to its viral load. Genome coverages were calculated from the consensus sequences generated by the RSVTyper pipeline. Whole genome sequences were defined as sequences with a genome coverage > 90%. The dashed line indicates 90% genome coverage.

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