Fig. 2 | Scientific Reports

Fig. 2

From: Paroxetine suppresses 27-hydroxycholesterol-induced responses in THP-1 human monocytic cells by regulating the AKT/mTORC1 pathway

Fig. 2

PRX suppresses MMP-9 activity. THP-1 cells were serum-starved as described in Fig. 1. and treated with 27OHChol (2 μg/mL) and the indicated concentrations of PRX for 48 h. (A) MMP-9 mRNA levels were measured by quantitative reverse transcription-PCR. Data were normalized to GAPDH and presented relative to control. (B) Gelatinolytic activity in culture supernatants was assessed by zymography. Clear zones indicate MMP-9 activity. Data represent mean ± SD of three independent biological replicates (n = 3). ###p < 0.001 vs control; ***p < 0.001 vs 27OHChol. Original full-length gels are presented in Supplementary Fig. 4.

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