Fig. 4 | Scientific Reports

Fig. 4

From: Paroxetine suppresses 27-hydroxycholesterol-induced responses in THP-1 human monocytic cells by regulating the AKT/mTORC1 pathway

Fig. 4

PRX suppresses phosphorylation of Akt, S6, and 4E-BP1. THP-1 cells were serum-starved and treated with 27OHChol (2 μg/mL) in the presence of PRX or rapamycin (Rapa) (0.01 μM). Total and phosphorylated forms of Akt, S6, and 4E-BP1 were analyzed by Western blotting at three post-treatment time points: 40 min (A), 1–4 h (B), and 48 h (C). Results are representative of three independent biological replicates (n = 3). The presented blots were cropped for clarity, and the corresponding original images are available in Supplementary Fig. 3.

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