Fig. 2
From: Tacrolimus modulates the PI3K AKT mTOR pathway in retinal epithelial cells under inflammatory stress
RT-qPCR validation of PI3K/AKT/mTOR-related mRNAs. Dot plots show relative expression (RQ = 2−ΔΔCt) of IL-2, MTOR, MAP2K2, COL1A1, and COL1A2 in H-RPE cells treated with LPS, tacrolimus, or their combination for 6 h, 12 h, and 24 h compared with control (C). Each dot represents one biological replicate (n = 3). Asterisks denote significant differences compared to control (p < 0.05). Genes abbreviation: IL-2, Interleukin 2; MTOR, Mechanistic Target of Rapamycin Kinase; MAP2K2, Mitogen-Activated Protein Kinase Kinase 2; COL1A1, Collagen Type I Alpha 1 Chain; COL1A2, Collagen Type I Alpha 2 Chain.
