Table 1 Primers sequences used to RT-qPCR.
From: Tacrolimus modulates the PI3K AKT mTOR pathway in retinal epithelial cells under inflammatory stress
mRNA | Starter | Sequence |
|---|---|---|
IL-2 | Forward | 5’-TCCTCAACTCCTGCCACAATG-3’ |
Reverse | 5’-TGTGAGCATCCTGGTGAGTT-3’ | |
MTOR | Forward | 5’-CCTGCCTTTGTCATGCCTTT-3’ |
Reverse | 5’-CTGGGTTTGGATCAGGGTCT-3’ | |
MAP2K2 | Forward | 5’-GGGACGTTCATCACCAACAC-3’ |
Reverse | 5’-CTTCAGGTAGTACAGCGCCT-3’ | |
COL1A1 | Forward | 5’- GCTACTACCGGGCTGATGAT-3’ |
Reverse | 5’- ACCAGTCTCCATGTTGCAGA-3’ | |
COL1A2 | Forward | 5’- GAGAGAGCGGTAACAAGGGT-3’ |
Reverse | 5’- GAAGACCACGAGAACCAGGA-3’ | |
ACTB | Forward | 5’-TCACCCACACTGTGCCCATCTACGA-3’ |
Reverse | 5’-CAGCGGAACCGCTCATTGCCAATGG-3’ | |
GAPDH | Forward | 5’- GGTGAAGGTCGGAGTCAACGGA-3’ |
Reverse | 5’- GAGGGATCTCGCTCCTGGAAGA-3’ |
