Table 3 Differential expression of selected mRNAs and their regulatory MicroRNAs in H-RPE cells treated with LPS or LPS with tacrolimus compared to Control.

From: Tacrolimus modulates the PI3K AKT mTOR pathway in retinal epithelial cells under inflammatory stress

mRNA

miRNA

Target score

LPS

Tacrolimus

LPS + tacrolimus

log2 fold change

H_6 vs. C

H_6 vs. C

H_6 vs. C

H_12 vs. C

H_24 vs. C

IL-2

hsa-miR-462

98

-2.19 ± 0.51

2.65 ± 0.34

3.17 ± 0.54

3.76 ± 0.22

3.89 ± 0.43

hsa-miR-27a-5p

94

-3.29 ± 0.42

3.14 ± 0.31

2.78 ± 0.76

3.54 ± 0.34

4.01 ± 0.45

hsa-miR-3163

90

-2.34 ± 0.43

2.87 ± 0.19

2.98 ± 0.71

2.89 ± 0.42

3.01 ± 0.16

MTOR

hsa-miR-3182

92

-2.87 ± 0.18

1.54 ± 0.81

3.45 ± 0.4

3.76 ± 0.91

3.18 ± 0.76

hsa-miR-1271-5p

97

-3.09 ± 0.33

2.01 ± 0.23

4.56 ± 0.65

3.76 ± 0.17

3.11 ± 0.12

COL1A1

hsa-miR-29a-3p

98

-3.98 ± 0.87

-1.01 ± 0.19

2.34 ± 0.81

2.76 ± 0.43

2.91 ± 0.34

COL1A2

90

  1. Data are presented as mean ± SD.
  2. IL-2, Interleukin 2; MTOR, Mechanistic Target of Rapamycin Kinase; MAP2K2, Mitogen-Activated Protein Kinase Kinase 2; COL1A1, Collagen Type I Alpha 1 Chain; COL1A2, Collagen Type I Alpha 2 Chain; LPS, ipopolysaccharide; C, control cell culture; FC, fold change; C: control culture; H_6, H_8, and H_24, time of exposure to the medicine. All listed changes are statistically significant compared to control (C) (p < 0.05).
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