Table 4 Time-Dependent changes in the concentration of selected proteins in H-RPE cell cultures treated with LPS, Tacrolimus, or their combination compared to Control.

From: Tacrolimus modulates the PI3K AKT mTOR pathway in retinal epithelial cells under inflammatory stress

Protein

C

LPS

Tacrolimus

LPS + tacrolimus

H_6

H_12

H_24

MAP2K2 [ng/mL]

1.55 ± 0.11

2.87 ± 0.12

1.99 ± 0.17

0.96 ± 0.08

0.76 ± 0.22

0.72 ± 0.12

COL1A1 [ng/mL]

10.98 ± 0.32

12.45 ± 0.23

7.87 ± 0.54

4.12 ± 0.43

2.84 ± 0.43

1.77 ± 0.18

COL1A2 [ng/mL]

21.53 ± 0.98

28.98 ± 1.23

20.11 ± 0.91

6.87 ± 0.66

6.55 ± 0.32

4.65 ± 0.43

MTOR [ng/mL]

2.60 ± 0.12

5.76 ± 0.17

3.11 ± 0.32

1.76 ± 0.77

1.11 ± 0.71

1.09 ± 0.23

IL-2 [pg/mL]

14.87 ± 1.32

23.87 ± 1.34

24.89 ± 2.34

28.90 ± 2.31

34.98 ± 3.43

32.82 ± 2.09

  1. Data are presented as mean ± SD IL-2, Interleukin 2; MTOR, Mechanistic Target of Rapamycin Kinase; MAP2K2, Mitogen-Activated Protein Kinase Kinase 2; COL1A1, Collagen Type I Alpha 1 Chain; COL1A2, Collagen Type I Alpha 2 Chain; LPS, lipopolisaccharide A; C, control cell culture; FC, fold change; C: control culture; H_6, H_8, and H_24, time of exposure to the medicine All listed changes are statistically significant compared to control (C) (p < 0.05).
Back to article page