Fig. 4
Effect of ex vivo DNase-1 Pre-incubation on patient mucus. (A) Representative fluorescence images of patient mucus stained for DNA (DAPI, blue) and MPO (red), illustrating extensive extracellular DNA coated with MPO. Pre-incubation with DNase-1 leads to marked degradation of these DNA structures. Scale bar = 40 μm. Excitation/emission wavelengths used: 375–407/420–450; 542–566/578–610. (B) Quantification of extracellular DNA degradation following DNase-1 pre-incubation, measured by fluorescence intensity (n = 5 per group).
