Fig. 1 | Scientific Reports

Fig. 1

From: Intergenerational effects of early life protein restriction on adipose tissue development as revealed by sheep transcriptomic analyses

Fig. 1

Overview of the RNA-Seq analysis pipeline. (a) Table showing the distribution of 16 samples categorized into two perirenal fatness level (KKCF, from kidney knob and channel fat) groups: High-KKCF (blue) and Low-KKCF (yellow). Each group includes offspring from mothers subjected to two prepubertal dietary conditions: Control (C, green) and Maternal Nutritional Protein Restriction (MNPR, red). (b) Bioinformatics workflow applied to RNA-Seq data, including mapping (STAR), quantification (RSEM), differential expression analysis (DEA; DESeq2) between High-KKCF and Low-KKCF independently for each maternal dietary condition (Control and MNPR), meta-analysis (metaRNASeq), and over-representation analysis (ORA) using clusterProfiler.

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