Table 2 Amounts of Buffer Mix, Enzyme Mix, and DEPC-treated water, added to the extracted RNA for cDNA synthesis according to Pars Tous Company’s instructions.

From: Investigating the expression changes of several key genes in prostate cancer cells under exposure to the ELF pulsed electromagnetic fields

Group

RNA mass, μg

RNA volume, μl

Buffer Mix, μl

Enzyme Mix, μl

DEPC-treated water, μl

Total volume, μl

1 (control)

0.097

1

10

2

7

20

2 (under EMF of 22.6 mT in 30 min)

0.041

2.36

10

2

5.64

20

3 (under EMF of 35 mT in 30 min)

0.048

2.02

10

2

5.98

20

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