Table 5 Master Mixes prepared for the studied genes and internal control genes according to the instructions of Pars Tous Company.
Gene name | Water volume, μl | Forward primer volume, μl | Reverse primer volume, μl | Syber Green mixture volume, μl | cDNA volume, μl | Total volume, μl |
|---|---|---|---|---|---|---|
GAPDH (internal control gene) | 7 | 1 | 1 | 10 | 1 | 20 |
BAX | 7 | 1 | 1 | 10 | 1 | 20 |
BCL-2 | 7 | 1 | 1 | 10 | 1 | 20 |
PTEN | 7 | 1 | 1 | 10 | 1 | 20 |
SNORD (internal control gene) | 8 | 0.5 | 0.5 | 10 | 1 | 20 |
MIR-21 | 8 | 0.5 | 0.5 | 10 | 1 | 20 |
