Fig. 2 | Scientific Reports

Fig. 2

From: Characterization of a phosphoinositide-binding protein containing a PHOX homology domain in the malaria parasite Plasmodium falciparum

Fig. 2

PfPX2-2xFKBP-GFP line generation and stage-specific expression in the asexual erythrocytic cycle. (A) Illustration of the single crossover recombination strategy employed for gene tagging using the SLI system. (B) PCR-based validation of tagging vector integration at the PfPX2 locus performed on parasite genomic DNA from a clonal line. Correct integration was assessed by amplifying junction regions (5′: P1/P2 primers; 3′: P3/P4 primers), while loss of the wild-type allele in PfPX2-2xFKBP-GFP parasites was confirmed using primers P1/P4. (C) Anti-GFP western blot on mixed-stage parasite protein extracts showing the proper expression of PfPX2-2xFKBP-GFP at the expected size of around 168 kDa. (D) Live microscopy showing that PfPX2-2xFKBP-GFP is expressed in trophozoite and schizont stages. Scale bar represents 5 µm. Blue: DAPI stained nucleus.

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